7-Alpha-Hydroxysteroid Dehydrogenase (HSD7a)

Wikipedia

Cloud-Clone

S429

PubMed

2007545

A bile acid-inducible NADP-linked 7 alpha-hydroxysteroid dehydrogenase (7 alpha-HSDH) from Clostridium sordellii ATCC 9714 was purified 310-fold by ion-exchange, gel filtration, and dye-ligand affinity chromatography.The N-terminal sequence was determined, and the corresponding oligonucleotides were synthesized and used to screen EcoRI and HindIII genomic digests of C. sordellii.The EcoRI fragment was shown to contain a truncated 7 alpha-HSDH gene, while the HindIII fragment contained the entire coding region. E. coli clones containing the HindIII insert expressed high levels of an NADP-linked 7 alpha-HSDH. Nucleotide sequence analyses suggest that the 7 alpha-HSDH is encoded by a monocistronic transcriptional unit, with DNA sequence elements resembling rho-independent terminators located in both the upstream and downstream flanking regions. The transcriptional start site was located by primer extension analysis.

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Sandwich ELISA for 7-Alpha-Hydroxysteroid Dehydrogenase (HSD7a)

Организм:

Человек

Артикул:

SES429Hu

Sandwich ELISA for 7-Alpha-Hydroxysteroid Dehydrogenase (HSD7a)

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